The goal of the Cell and Molecular Metabolism Core is to provide support and resources for metabolic phenotyping and mechanistic studies though animal and cell-based approaches to advance understanding of metabolic pathways contributing to health and disease. Cellular metabolism is central to conveying environmental cues, providing fuel sources, and maintaining energy balance. Disruption or imbalances in cell metabolism impinge upon cellular function and underlie cellular stress associated with obesity and nutrient-related disease pathologies.
Services
The Core will provide members with access to technology and services for mouse metabolic phenotyping, for comprehensive analysis of cellular respiration, metabolomics, and metabolite tracing, and to support in vitro genetic studies.
- Glucose and insulin tolerance tests
- MRI—assessment of fat distribution
- Seahorse XF analyzer—24- and 96-well systems for measuring oxygen consumption rates and extracellular acidification rate to assess mitochondrial and glycolytic metabolism, respectively
- ELISAs
- Metabolomics— GC- and LC-MS based measurement of lipids, TCA and glycolysis metabolites, amino acids
- Metabolomic flux analyses
- In vitro assays—glucose uptakes assays, insulin signaling, lipolysis
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