Define the impact of HFDs on macrophage clearance of apoptotic neutrophils in response to intestinal damage.
Macrophage recognition of apoptotic neutrophils is key to their production of pro-tissue repair responses to tissue injury. Using mouse models and in vitro studies, we seek to understand how HFDs impact macrophage pro-tissue repair functions in response to damage stimuli such as apoptotic neutrophils.
Define the impact of HFD on human macrophage responses to intestinal injury in a human intestinal epithelial-macrophage co-culture system.
Human macrophage recognition of apoptotic neutrophils is key to the inductions of tissue repair functions. Using a novel human enteroid-macrophage co-culture system, we seek to understand how HFDs impact macrophage pro-tissue repair functions of human macrophages and the resulting impact on intestinal epithelial healing.
Determine the impact of unsaturated and saturated fatty acids on macrophage responses to apoptotic neutrophils and the impact intestinal epithelial healing.
The lipid composition of HFD is complex, and it is critical to understand how the overall diet composition and specific lipid components impact intestinal repair. We seek to define how specific lipid components of the diet affect macrophage responses to intestinal damage signals.
