Baylor College of Medicine

Michael A Mancini, Ph.D.

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Mancini

Michael A Mancini, Ph.D.

Professor and Core Director

Email

mancini@bcm.edu

Positions

Professor and Core Director: Baylor College of Medicine
Houston, Texas United States

Addresses

BCM-Cullen Building (Office)
: Room: BCMC-119A
Houston, TX 77030
United States
Phone: (713) 798-8952
mancini@bcm.edu
https://www.bcm.edu/people/view/b207808c-ffed-11e2-be68-080027880ca6/53f64f3f-a3d4-4102-8eaa-1e1e0a1612dc

BCM-Cullen Building (Lab)
: Room: BCMC-119A
Houston, TX 77030
United States
Phone: (713) 798-8952
mancini@bcm.edu
https://www.bcm.edu/people/view/b207808c-ffed-11e2-be68-080027880ca6/53f64f3f-a3d4-4102-8eaa-1e1e0a1612dc

Education

PhD from University Of Texas Health Science Center: 01/1991 - San Antonio, TX United States; Cellular and Structural Biology

Honors & Awards

Professor: My group's interests revolve around single cell analysis of gene expression, primarily through native or engineered estrogen receptor model systems. Model systems are designed for use with quantitative, high content analysis using a wide range of imaging platforms, ranging from high throughput microscopy to multiple super resolution platforms (SIM, STED, STORM) followed by image analysis, data mining and classifications approaches, including deep learning and neural networks. Current funding centers around use of the above approaches to detect/quantify/classify endocrine disrupting chemicals (EDCs) in flood waters associated with Galveston Bay and the Houston Ship Channel.; Baylor College of Medicine (05/1995)

Professional Interests

Single cell analysis of gene regulation

Websites

VIICTR Publications List

Selected Publications

Nazareth LV, Stenoien DL, Bingman WE, James AJ, Wu C, Zhang Y, Edwards DP, Mancini M, Marcelli M, Lamb DJ, Weigel NL "A C619Y mutation in the human androgen receptor causes inactivation and mislocalization of the receptor with concomitant sequestration of SRC-1 (steroid receptor coactivator 1)." Mol. Endocrinol.. 1999 Dec;13(12):2065-75. Pubmed PMID: 10598582
Slattery SD, Newberg JY, Szafran AT, Hall RM, Brinkley BR, Mancini MA "A framework for image-based classification of mitotic cells in asynchronous populations.." Assay Drug Dev Technol. 2012 Apr;10(2):161-78. Pubmed PMID: 22084958
Berno V, Amazit L, Hinojos C, Zhong J, Mancini MG, Sharp ZD, Mancini MA "Activation of estrogen receptor-alpha by E2 or EGF induces temporally distinct patterns of large-scale chromatin modification and mRNA transcription.." PLoS ONE. 2008;3(5):e2286. Pubmed PMID: 18509470
Mancini MA, Chatterjee B, Roy AK "Age-dependent reversal of the lobular distribution of androgen-inducible alpha 2u globulin and androgen-repressible SMP-2 in rat liver.." J. Histochem. Cytochem.. 1991 Apr;39(4):401-5. Pubmed PMID: 1706374

Mancini MA, Majumdar D, Chatterjee B, Roy AK "Alpha 2u-globulin in modified sebaceous glands with pheromonal functions: localization of the protein and its mRNA in preputial, meibomian, and perianal glands.." J. Histochem. Cytochem.. 1989 Feb;37(2):149-57. Pubmed PMID: 2463299
Hartig SM, He B, Long W, Buehrer BM, Mancini MA "Homeostatic levels of SRC-2 and SRC-3 promote early human adipogenesis.." J. Cell Biol.. 2011 Jan 10;192(1):55-67. Pubmed PMID: 21220509
Van Hooser A, Goodrich DW, Allis CD, Brinkley BR, Mancini MA "Histone H3 phosphorylation is required for the initiation, but not maintenance, of mammalian chromosome condensation.." J. Cell. Sci.. 1998 Dec;111:3497-506. Pubmed PMID: 9811564
Berno V, Hinojos CA, Amazit L, Szafran AT, Mancini MA "High-resolution, high-throughput microscopy analyses of nuclear receptor and coregulator function.." Meth. Enzymol.. 2006;414:188-210. Pubmed PMID: 17110193
Ashcroft FJ, Newberg JY, Jones ED, Mikic I, Mancini MA "High content imaging-based assay to classify estrogen receptor-a ligands based on defined mechanistic outcomes.." Gene. 2011 May 15;477(41276):42-52. Pubmed PMID: 21256200
Giordano C, Cui Y, Barone I, Ando S, Mancini MA, Berno V, Fuqua SA "Growth factor-induced resistance to tamoxifen is associated with a mutation of estrogen receptor alpha and its phosphorylation at serine 305.." Breast Cancer Res. Treat.. 2010 Jan;119(1):71-85. Pubmed PMID: 19205871
Van Hooser AA, Mancini MA, Allis CD, Sullivan KF, Brinkley BR "The mammalian centromere: structural domains and the attenuation of chromatin modeling.." FASEB J.. 1999 Dec;13:S216-20. Pubmed PMID: 10619130
Zwart W, de Leeuw R, Rondaij M, Neefjes J, Mancini MA, Michalides R "The hinge region of the human estrogen receptor determines functional synergy between AF-1 and AF-2 in the quantitative response to estradiol and tamoxifen.." J. Cell. Sci.. 2010 Apr 15;123:1253-61. Pubmed PMID: 20332105
Contreras A, Hale TK, Stenoien DL, Rosen JM, Mancini MA, Herrera RE "The dynamic mobility of histone H1 is regulated by cyclin/CDK phosphorylation.." Mol. Cell. Biol.. 2003 Dec;23(23):8626-36. Pubmed PMID: 14612406
Lee WH, Xu Y, Hong F, Durfee T, Mancini MA, Ueng YC, Chen PL, Riley D "The corral hypothesis: a novel regulatory mode for retinoblastoma protein function.." Cold Spring Harb. Symp. Quant. Biol.. 1994;59:97-107. Pubmed PMID: 7587136
Herrmann CH, Mancini MA "The Cdk9 and cyclin T subunits of TAK/P-TEFb localize to splicing factor-rich nuclear speckle regions.." J. Cell. Sci.. 2001 Apr;114:1491-503. Pubmed PMID: 11282025
Zhu X, Chang KH, He D, Mancini MA, Brinkley WR, Lee WH "The C terminus of mitosin is essential for its nuclear localization, centromere/kinetochore targeting, and dimerization.." J. Biol. Chem.. 1995 Aug 18;270(33):19545-50. Pubmed PMID: 7642639
Durfee T, Mancini MA, Jones D, Elledge SJ, Lee WH "The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing.." J. Cell Biol.. 1994 Nov;127(3):609-22. Pubmed PMID: 7525595
Mediwala SN, Sun H, Szafran AT, Hartig SM, Sonpavde G, Hayes TG, Thiagarajan P, Mancini MA, Marcelli M "The activity of the androgen receptor variant AR-V7 is regulated by FOXO1 in a PTEN-PI3K-AKT-dependent way.." Prostate. 2013 Feb 15;73(3):267-77. Pubmed PMID: 22821817
Magnuson VL, Young M, Schattenberg DG, Mancini MA, Chen DL, Steffensen B, Klebe RJ "The alternative splicing of fibronectin pre-mRNA is altered during aging and in response to growth factors.." J. Biol. Chem.. 1991 Aug 5;266(22):14654-62. Pubmed PMID: 1713586
Chatterjee B, Song CS, Jung MH, Chen S, Walter CA, Herbert DC, Weaker FJ, Mancini MA, Roy AK "Targeted overexpression of androgen receptor with a liver-specific promoter in transgenic mice.." Proc. Natl. Acad. Sci. U.S.A.. 1996 Jan 23;93(2):728-33. Pubmed PMID: 8570624
Stenoien DL, Mancini MG, Patel K, Allegretto EA, Smith CL, Mancini MA "Subnuclear trafficking of estrogen receptor-alpha and steroid receptor coactivator-1.." Mol. Endocrinol.. 2000 Apr;14(4):518-34. Pubmed PMID: 10770489
Mancini MG, Liu B, Sharp ZD, Mancini MA "Subnuclear partitioning and functional regulation of the Pit-1 transcription factor.." J. Cell. Biochem.. 1999 Mar 1;72(3):322-38. Pubmed PMID: 10022514
Stenoien DL, Simeoni S, Sharp ZD, Mancini MA "Subnuclear dynamics and transcription factor function.." J. Cell. Biochem. Suppl.. 2000;99-106. Pubmed PMID: 11389538
Long W, Yi P, Amazit L, LaMarca HL, Ashcroft F, Kumar R, Mancini MA, Tsai SY, Tsai MJ, O'Malley BW "SRC-3Delta4 mediates the interaction of EGFR with FAK to promote cell migration.." Mol. Cell. 2010 Feb 12;37(3):321-32. Pubmed PMID: 20159552
Mancini MA, Song CS, Rao TR, Chatterjee B, Roy AK "Spatio-temporal expression of estrogen sulfotransferase within the hepatic lobule of male rats: implication of in situ estrogen inactivation in androgen action.." Endocrinology. 1992 Sep;131(3):1541-6. Pubmed PMID: 1380444
Murty CV, Sarkar FH, Mancini MA, Roy AK "Sex-independent synthesis of alpha 2u-globulin and its messenger ribonucleic acid in the rat preputial gland: biochemical and immunocytochemical analyses.." Endocrinology. 1987 Sep;121(3):1000-5. Pubmed PMID: 2441975
Roy AK, Sarkar FH, Nag AC, Mancini MA "Role of cytodifferentiation and cell-cell communication in the androgen dependent expression of alpha 2u globulin gene in rat liver.." Prog. Clin. Biol. Res.. 1986;226:401-15. Pubmed PMID: 2433699
Amazit L, Pasini L, Szafran AT, Berno V, Wu RC, Mielke M, Jones ED, Mancini MG, Hinojos CA, O'Malley BW, Mancini MA "Regulation of SRC-3 intercompartmental dynamics by estrogen receptor and phosphorylation.." Mol. Cell. Biol.. 2007 Oct;27(19):6913-32. Pubmed PMID: 17646391
Marcelli M, Stenoien DL, Szafran AT, Simeoni S, Agoulnik IU, Weigel NL, Moran T, Mikic I, Price JH, Mancini MA "Quantifying effects of ligands on androgen receptor nuclear translocation, intranuclear dynamics, and solubility.." J. Cell. Biochem.. 2006 Jul 1;98(4):770-88. Pubmed PMID: 16440331
Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino GN, Marcelli M, Weigel NL, Mancini MA "Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone.." Hum. Mol. Genet.. 1999 May;8(5):731-41. Pubmed PMID: 10196362
Prigozhina NL, Zhong L, Hunter EA, Mikic I, Callaway S, Roop DR, Mancini MA, Zacharias DA, Price JH, McDonough PM "Plasma membrane assays and three-compartment image cytometry for high content screening.." Assay Drug Dev Technol. 2007 Feb;5(1):29-48. Pubmed PMID: 17355198
Hinojos CA, Sharp ZD, Mancini MA "Molecular dynamics and nuclear receptor function.." Trends Endocrinol. Metab..;16(1):41616. Pubmed PMID: 15620544
Hendzel MJ, Wei Y, Mancini MA, Van Hooser A, Ranalli T, Brinkley BR, Bazett-Jones DP, Allis CD "Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation.." Chromosoma. 1997 Nov;106(6):348-60. Pubmed PMID: 9362543
Stenoien DL, Nye AC, Mancini MG, Patel K, Dutertre M, O'Malley BW, Smith CL, Belmont AS, Mancini MA "Ligand-mediated assembly and real-time cellular dynamics of estrogen receptor alpha-coactivator complexes in living cells.." Mol. Cell. Biol.. 2001 Jul;21(13):4404-12. Pubmed PMID: 11390668
García-Becerra R, Berno V, Ordaz-Rosado D, Sharp ZD, Cooney AJ, Mancini MA, Larrea F "Ligand-induced large-scale chromatin dynamics as a biosensor for the detection of estrogen receptor subtype selective ligands.." Gene. 2010 Jun 15;458(41276):37-44. Pubmed PMID: 20347019
Stenoien DL, Mielke M, Mancini MA "Intranuclear ataxin1 inclusions contain both fast- and slow-exchanging components.." Nat. Cell Biol.. 2002 Oct;4(10):806-10. Pubmed PMID: 12360291
Sharp ZD, Stenoien DL, Mancini MG, Ouspenski II, Mancini MA "Inactivating Pit-1 mutations alter subnuclear dynamics suggesting a protein misfolding and nuclear stress response.." J. Cell. Biochem.. 2004 Jul 1;92(4):664-78. Pubmed PMID: 15211565
Stenoien D, Sharp ZD, Smith CL, Mancini MA "Functional subnuclear partitioning of transcription factors.." J. Cell. Biochem.. 1998 Aug 1;70(2):213-21. Pubmed PMID: 9671227
Stenoien DL, Patel K, Mancini MG, Dutertre M, Smith CL, O'Malley BW, Mancini MA "FRAP reveals that mobility of oestrogen receptor-alpha is ligand- and proteasome-dependent.." Nat. Cell Biol.. 2001 Jan;3(1):15-23. Pubmed PMID: 11146621
Hartig SM, He B, Newberg JY, Ochsner SA, Loose DS, Lanz RB, McKenna NJ, Buehrer BM, McGuire SE, Marcelli M, Mancini MA "Feed-forward inhibition of androgen receptor activity by glucocorticoid action in human adipocytes.." Chem. Biol.. 2012 Sep 21;19(9):1126-41. Pubmed PMID: 22999881
Sharp ZD, Mancini MG, Hinojos CA, Dai F, Berno V, Szafran AT, Smith KP, Lele TP, Lele TT, Ingber DE, Mancini MA "Estrogen-receptor-alpha exchange and chromatin dynamics are ligand- and domain-dependent.." J. Cell. Sci.. 2006 Oct 1;119:4101-16. Pubmed PMID: 16968748
Dong S, Qiu J, Stenoien DL, Brinkley WR, Mancini MA, Tweardy DJ "Essential role for the dimerization domain of NuMA-RARalpha in its oncogenic activities and localization to NuMA sites within the nucleus.." Oncogene. 2003 Feb 13;22(6):858-68. Pubmed PMID: 12584566
Mancini MA, He D, Ouspenski II, Brinkley BR "Dynamic continuity of nuclear and mitotic matrix proteins in the cell cycle.." J. Cell. Biochem.. 1996 Aug;62(2):158-64. Pubmed PMID: 8844395
Stenoien DL, Sen S, Mancini MA, Brinkley BR "Dynamic association of a tumor amplified kinase, Aurora-A, with the centrosome and mitotic spindle.." Cell Motil. Cytoskeleton. 2003 Jun;55(2):134-46. Pubmed PMID: 12740874
Narayanan R, Yepuru M, Szafran AT, Szwarc M, Bohl CE, Young NL, Miller DD, Mancini MA, Dalton JT "Discovery and mechanistic characterization of a novel selective nuclear androgen receptor exporter for the treatment of prostate cancer.." Cancer Res.. 2010 Jan 15;70(2):842-51. Pubmed PMID: 20068182
McDonough PM, Maciejewski-Lenoir D, Hartig SM, Hanna RA, Whittaker R, Heisel A, Nicoll JB, Buehrer BM, Christensen K, Mancini MG, Mancini MA, Edwards DP, Price JH "Differential Phosphorylation of Perilipin 1A at the Initiation of Lipolysis Revealed by Novel Monoclonal Antibodies and High Content Analysis.." PLoS ONE. 2013;8(2):e55511. Pubmed PMID: 23405163
Kazansky AV, Kabotyanski EB, Wyszomierski SL, Mancini MA, Rosen JM "Differential effects of prolactin and src/abl kinases on the nuclear translocation of STAT5B and STAT5A.." J. Biol. Chem.. 1999 Aug 6;274(32):22484-92. Pubmed PMID: 10428824
Ho TH, Savkur RS, Poulos MG, Mancini MA, Swanson MS, Cooper TA "Colocalization of muscleblind with RNA foci is separable from mis-regulation of alternative splicing in myotonic dystrophy.." J. Cell. Sci.. 2005 Jul 1;118:2923-33. Pubmed PMID: 15961406
Bolt MJ, Stossi F, Newberg JY, Orjalo A, Johansson HE, Mancini MA "Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses.." Nucleic Acids Res.. 2013 Apr 1;41(7):4036-48. Pubmed PMID: 23444138
Zhu X, Mancini MA, Chang KH, Liu CY, Chen CF, Shan B, Jones D, Yang-Feng TL, Lee WH "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression.." Mol. Cell. Biol.. 1995 Sep;15(9):5017-29. Pubmed PMID: 7651420
Cummings CJ, Mancini MA, Antalffy B, DeFranco DB, Orr HT, Zoghbi HY "Chaperone suppression of aggregation and altered subcellular proteasome localization imply protein misfolding in SCA1.." Nat. Genet.. 1998 Jun;19(2):148-54. Pubmed PMID: 9620770
Murty CV, Mancini MA, Chatterjee B, Roy AK "Changes in transcriptional activity and matrix association of alpha 2u-globulin gene family in the rat liver during maturation and aging.." Biochim. Biophys. Acta. 1988 Jan 25;949(1):27-34. Pubmed PMID: 2446666
Unni E, Sun S, Nan B, McPhaul MJ, Cheskis B, Mancini MA, Marcelli M "Changes in androgen receptor nongenotropic signaling correlate with transition of LNCaP cells to androgen independence.." Cancer Res.. 2004 Oct 1;64(19):7156-68. Pubmed PMID: 15466214
Sarkar FH, Mancini MA, Nag AC, Roy AK "Cellular interactions in the hormonal induction of alpha 2u-globulin in rat liver.." J. Endocrinol.. 1986 Nov;111(2):205-8. Pubmed PMID: 2432143
Uray IP, Rodenberg JM, Bissonnette RP, Brown PH, Mancini MA "Cancer-Preventive Rexinoid Modulates Neutral Lipid Contents of Mammary Epithelial Cells through a Peroxisome Proliferator-Activated Receptor ?-Dependent Mechanism.." Mol. Pharmacol.. 2012 Feb;81(2):228-38. Pubmed PMID: 22053058
Chen W, Chang B, Saha P, Hartig SM, Li L, Reddy VT, Yang Y, Yechoor V, Mancini MA, Chan L "Berardinelli-seip congenital lipodystrophy 2/seipin is a cell-autonomous regulator of lipolysis essential for adipocyte differentiation.." Mol. Cell. Biol.. 2012 Mar;32(6):1099-111. Pubmed PMID: 22269949
Hartig SM, Newberg JY, Bolt MJ, Szafran AT, Marcelli M, Mancini MA "Automated microscopy and image analysis for androgen receptor function.." Methods Mol. Biol.. 2011;776:313-31. Pubmed PMID: 21796534
Slattery SD, Mancini MA, Brinkley BR, Hall RM "Aurora-C kinase supports mitotic progression in the absence of Aurora-B.." Cell Cycle. 2009 Sep 15;8(18):2984-94. Pubmed PMID: 19713763
Das A, Frank RN, Weber ML, Kennedy A, Reidy CA, Mancini MA "ATP causes retinal pericytes to contract in vitro.." Exp. Eye Res.. 1988 Mar;46(3):349-62. Pubmed PMID: 3258246
Szafran AT, Hartig S, Sun H, Uray IP, Szwarc M, Shen Y, Mediwala SN, Bell J, McPhaul MJ, Mancini MA, Marcelli M "Androgen receptor mutations associated with androgen insensitivity syndrome: a high content analysis approach leading to personalized medicine.." PLoS ONE. 2009;4(12):e8179. Pubmed PMID: 20011049
Szafran AT, Sun H, Hartig S, Shen Y, Mediwala SN, Bell J, McPhaul MJ, Mancini MA, Marcelli M "Androgen receptor mutations associated with androgen insensitivity syndrome: a high content analysis approach leading to personalized medicine.." Adv. Exp. Med. Biol.. 2011;707:63-5. Pubmed PMID: 21691957
Song CS, Rao TR, Demyan WF, Mancini MA, Chatterjee B, Roy AK "Androgen receptor messenger ribonucleic acid (mRNA) in the rat liver: changes in mRNA levels during maturation, aging, and calorie restriction.." Endocrinology. 1991 Jan;128(1):349-56. Pubmed PMID: 1986927
Szafran AT, Szwarc M, Marcelli M, Mancini MA "Androgen receptor functional analyses by high throughput imaging: determination of ligand, cell cycle, and mutation-specific effects.." PLoS ONE. 2008;3(11):e3605. Pubmed PMID: 18978937
Nye AC, Rajendran RR, Stenoien DL, Mancini MA, Katzenellenbogen BS, Belmont AS "Alteration of large-scale chromatin structure by estrogen receptor.." Mol. Cell. Biol.. 2002 May;22(10):3437-49. Pubmed PMID: 11971975
Mancini MA, Shan B, Nickerson JA, Penman S, Lee WH "The retinoblastoma gene product is a cell cycle-dependent, nuclear matrix-associated protein.." Proc. Natl. Acad. Sci. U.S.A.. 1994 Jan 4;91(1):418-22. Pubmed PMID: 8278403
Boonyaratanakornkit V, McGowan E, Sherman L, Mancini MA, Cheskis BJ, Edwards DP "The role of extranuclear signaling actions of progesterone receptor in mediating progesterone regulation of gene expression and the cell cycle.." Mol. Endocrinol.. 2007 Feb;21(2):359-75. Pubmed PMID: 17138644
Chatterjee B, Mancini MA, Roy AK "The senescence marker protein (SMP-2) of the rat liver: purification, immunochemical characterization and age-dependent regulation.." Biochim. Biophys. Acta. 1990 May 16;1034(2):162-9. Pubmed PMID: 2354191

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Funding

Center for Advanced Microscopy and Image Informatics

Skills

Michael A. Mancini