NMR and Drug Metabolism Core Technology & Instrumentation

The hAvance III HD 800 MHz Spectrometer operates three RF channels plus deuterium using a quadruple resonance inverse detection QCI CryoProbe^TM (5 mm sample diameter) equipped with z-gradients. The system allows very high sensitivity observation of ¹H or ¹⁹F with ¹⁵N, ³¹P and ¹³C decoupling; alternatively, it supports high sensitivity ¹³C observation with ¹H and ¹⁵N decoupling. The SampleJet dual-mode automated sample changer accepts a mix of tube diameters (1, 1.7, 3, and 5 mm) for either standard 7” tubes or 4” SampleJet tubes in up to five racks of 96 samples.

The Avance III HD 600 MHz Spectrometer operates two RF channels (plus deuterium) using an inverse triple resonance probe (5 mm sample diameter, ¹H observe, ¹³C and ¹⁵N decoupling, ²H lock) with automatic tuning and matching and an actively shielded z-gradient coil. The SampleXpress™ sample changer with bar-code reader supports automation of up to 60 sample tubes (1.7 mm to 5 mm ODs; 100 mm to 228 mm lengths).

The Agilent 6490 Triple Quadrupole Mass Spectrometer (QQQ MS) coupled to an Agilent 1290 Infinity LC System (HPLC) operates in both positive and negative modes with electrospray ionization. The system has been widely used for microsomal stability screening, drug metabolism, and pharmacokinetics. The instrument allows quantitative and specific determination of the concentrations of drugs or other compounds in various biomaterials (serum, tissue, urine etc.) using the multiple reaction monitoring (MRM) method.